Biology 301--Cell & Molecular Biology

Spring 2008

Dr. William Hixon

 

Lecture Schedule

Laboratory Schedule

Powerpoint Lectures

 The purpose of this course is to examine in depth the structure and function of cells.  The scope of this examination will be from the basic building blocks of biological molecules to the production, activities, and evolution of the living structure.  Along the way, we will become acquainted with some of the techniques used to reveal the structure and function of cells.

 

Class text:  Becker, W.M., L.J. Kleinsmith and J. Hardin.  2006.  The World of the Cell, sixth edition.  The Benjamin/Cummings Publishing Co., Inc.

 

Office hours:  my office is located in Rm 209, Lewis Hall.  Feel free to stop by at any time or call to make an appointment.  My office phone number is 333-6180; my home phone number is 355-8781 (please use it with discretion); email address is HixonWilliamG at sau.edu. Official office hours will be announced in class and posted on my office door.

 

Attendance:  I do not monitor attendance in lecture, but attendance in lab and at exams is mandatory.  I recommend that you attend lecture because some material presented in class is not in the book and the discussion of concepts in class should aid your comprehension of the material.   Additionally, if your final grade is borderline, your attendance and participation in class will weigh in your favor.

 

Grading:  Your final grade will be the result of accumulated points.
                        Exams 1-5 (60 pts each)          ----->  300 pts
                        Lab grade                                ----->  160 pts

                        Attendance/Participation         ----->    40 pts
                        Total                                        ------> 500 pts

 

Your lab grade will be derived as follows:
            Lab Report #1A                                                            40 pts
            Lab Report #1B                                                             60 pts
            Lab Report #2                                                               60 pts
            Total                                                                            160 pts

 

NOTE:  Lab reports turned in late will receive a 10% reduction in score for each day past due.

 

Approximate letter grade values are:
            A  = 450+ pts                                                              C  = 335-374 pts
            B+ = 425-449 pts                                                        D  = 250-334 pts
            B  = 400-424 pts                                                         F  = <250 pts
            C+ = 375-399 pts

 

I strongly encourage each of you to obtain assistance from me at any time during the semester that you are having difficulty with the material.  Do not wait until you are way over your head to decide to do something about it.

 

Students with Disabilities:  :  Students with disabilities who believe that they may need accommodations in this class are encouraged to contact the Office of Services for Students with Disabilities at 333-6275 as soon as possible to better ensure that such accommodations are implemented in a timely fashion.

 

Academic Honesty:  All work submitted (exams and lab reports) are required to be entirely your own unless announced otherwise.  If you have any doubts as to the appropriateness of your actions, please do not hesitate to check with me.  I also recommend that you consult the college catalog regarding the institution’s policy on academic dishonesty.

 

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ANTICIPATED LECTURE SCHEDULE

 

  Dates                        Topic                                                                                 Readings

1/16                             Overview                                                                              Ch. 1

1/18                             Cell Chemistry                                                                      Ch. 2

1/21                             NO CLASS

1/23                             Macromolecules                                                                    Ch. 3

1/25                             Macromolecules                                                                    Ch. 3

1/28                             Cell Structure                                                                        Ch. 4

1/30                             Bioenergetics                                                                        Ch. 5

2/1                               Bioenergetics                                                                        Ch. 5

2/4                                  EXAM 1

 

2/6                               Enzymes                                                                                Ch. 6    

2/8                               Membranes                                                                            Ch. 7

2/11                             Membranes                                                                            Ch. 7

2/13                             Membrane Transport                                                             Ch. 8

2/15                             Glycolysis & Fermentation                                                    Ch. 9

2/18                             Aerobic Respiration                                                             Ch. 10

2/20                             Aerobic Respiration                                                             Ch. 10

2/22                                EXAM 2

 

2/25                             Photosynthesis                                                                      Ch. 11

2/27                             Intracellular Compartments                                                  Ch. 12   

2/29                             Intracellular Compartments                                                  Ch. 12

3/3-3/9                               SPRING BREAK (ENJOY)

3/10                             Electrical Signals                                                                 Ch. 13

3/12                             Electrical Signals                                                                 Ch. 13

3/14                             Chemical Signals                                                                  Ch. 14

3/17                             Chemical Signals                                                                  Ch. 14

3/19                                EXAM 3                                                                                

 

3/21-3/24                    NO CLASS—EASTER BREAK

3/26                             Cytoskeleton                                                                         Ch. 15

3/28                             Cytoskeleton                                                                         Ch. 15

3/31                             Motility & Contractility                                                        Ch. 16

4/2                               Motility & Contractility                                                        Ch. 16

4/4                               DNA & Chromosomes                                                         Ch. 18

4/7                               DNA & Chromosomes                                                         Ch. 18

4/9                               The Cell Cycle (pp. 554-6, 586-600)                                   Ch. 19

4/11                             Genetic Engineering (pp. 633-46)                                        Ch. 20

4/14                                EXAM 4                                                                                

 

4/16                             Genetic Code & Transcription                                             Ch. 21

4/18                             Genetic Code & Transcription                                             Ch. 21

4/21                             Protein Synthesis & Sorting                                                  Ch. 22

4/23                             Protein Synthesis & Sorting                                                  Ch. 22

4/25                             Gene Regulation                                                                   Ch. 23

4/28                             Gene Regulation                                                                   Ch. 23

4/30                             Cancer                                                                                  Ch. 24

5/2                               Cancer                                                                                  Ch. 24

 

FINAL EXAM:   Friday, May 9th, 10:00-11:50

 

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Laboratory Schedule

 

Note:  I may change some of the labs if preparation time allows.  I am considering some new exercises.  However, the experiments that require a lab report will not change, nor will the related deadlines.

 

You will usually be working with a lab partner.  Therefore attendance at all laboratory sessions is mandatory unless I have excused you beforehand. Each unexcused absence will result in the deduction of 10 pts from your attendance/participation score.  The lab will be held in Rm 215.  There is no lab manual; assignments will be distributed during either the preceding lab session or lecture.  The purpose of the laboratory is to introduce you to techniques used in cell and molecular biology, and to give you practice in deriving and reporting experimental results.  Please show up on time and bring a calculator to lab with you.

 

Date                 Topic

1/23            Introduction to the Lab:  Basic Techniques

1/30            Experimental Design

2/6              Membrane Permeability* (Spectrometry)

2/13            Discussion

2/20            Cell Fractionation (Centrifugation) [Lab Report #1A Due @ 1PM]

2/27            Organelles (Microscopy)

                                      Spring Break

3/12            Molecular Biology Databases

3/19            Contractility (Microscopy) [Lab Report #1B Due @ 1PM]

3/26            Tubulin Isolation (SDS-PAGE)

4/2              Restriction Mapping (DNA Agarose Gel Electrophoresis)

4/9              Enzyme Induction* (Spectrometry)
4/16            Discussion 

4/23            Salivary Antigens (Immunochemistry) [Lab Report #2 Due @ 1PM]

4/30            Summary & Evaluation

 

* Full Laboratory Reports